CGC Strain BC5344

Number:
BC5344
Species:
Caenorhabditis elegans
Genotype:
sEx414 [C27A12 (I) + pCes1943[rol-6(su1006)]].
Description:
sEx414 [C27A12 (I) + pCes1943[rol-6(su1006)]]. 2.8 ng/ul C27A12 + 100 ng/ul pCes1943[rol-6(su1006dm)] injected into hermaphrodite from BC842 (N2 male strain). pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants.
Mutagen:
Outcrossed:
Reference:
CGC #2901 Janke DL;Schein JE;Ha T;Franz NW;O'Neil NJ;Vatcher GP;Stewart HI;Kuervers LM;Baillie DL;Rose AM Interpreting a sequenced genome: Toward a cosmid transgenic library of Caenorhabditis elegans. Genome Research 7: 974-985 1997
Made_by:
Diana Janke
Received:
02/17/98 from Baillie D, Simon Fraser University, Burnaby, BC

Leon Avery (Leon@eatworms.swmed.edu)
Last modified: Wed Sep 24 07:15:03 2003